Coding

Part:BBa_K1334015:Design

Designed by: Qiushuang Wu   Group: iGEM14_WHU-China   (2014-09-27)


Formate dehydrogenase(FDH); from Candida boidinii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 316
    Illegal AgeI site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence contains a Pst1 site,after point mutation,we removed the site.


Source

The sequence was got from NCBI,then we PCR from the genome of Candida boidinii.The strain of Candida boidinii we use was bought from China Center of Industrial Culture Collection (CICC),the number in CICC is 33028.Professor Fang Peng and her team member Jiang Li help us get the genome so we can use the genome not the yeast itself to complete our design.

References